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In Haberkern et al. (2019) we compare fixation behavior of head-fixed walking flies in 1D and 2D virtual environments. Here we provide additional data on fixation behavior across different wild type genotypes. Further, we show how experimental conditions and preparation of flies before the experiment can influence the behavioral phenotype. We compare tested three wild type genotypes,:
- DL: Wild type strain from the Dickinson laboratory
- WTB: Isogenic wild type Berlin strain
- WTB hybrid: Flies generated by crossing WTB virgins with males from an enhancerless “empty GAL4” line
We chose the WTB flies because this genotype has and DL strains because they have been used in many numerous previous publications on various walking navigational behaviors. WTB hybrid flies were chosen to approximate the genotypes used in optogenetic activation experiments, where an effector line with WTB background is crossed to GAL4 driver lines with variable genetic backgrounds.
Extended data on fixation behavior
Fixation behavior across sexes and genotypes (A-E) Comparison of fixation behavior in WTB, WTB hybrid and DL flies measured at high room temperature (30 ?C) and with wings cut. (A) Fixation plots of unimodal fixation for female flies. These "fixation plots” illustrate the angular location of the fixation peak and the fixation strength based on von Mises fits. The location parameter (µ) is plotted on the circumferential axis and the shape parameter (k) on the radial axis. Markers (empty or filled circle) indicate categorization of trial based on fit. The grey arrow points toward the frontal position in the fly’s field of view. Grey shading marks the part of the field of view that is not covered by the panoramic screen. Visualization as in Fig. 3 F of Haberkern et al (2019). (B) Fixation plots of bimodal for female flies. Note different axis scale in bright and dark scenes. Visualization as in Fig. 3 G of Haberkern et al (2019). (C) Fraction of male and female flies that walked for at least 20 % of the trial time across the four trials. (D, E) Fraction of flies showing unimodal (D) and bimodal (E) fixation. (F-H) Same as (C-E), but for different experimental protocols: Flies were either wing cut and measured at high (30 ?C) or low (25 ?C) room temperature or wing glued and measured at high (30 ?C) room temperature.
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