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Make a shutter pulse (Width=100ms; Amp=5000 (note - this corresponds to 5 V); Delay=100) in a new Shutter pulse set and select it in the stimulator.
Running multiple programs simultaneously
Now select 'External' for the stimulator, acquirer, and ephys programs. All progrmas will now wait for an external trigger. Select Loop on the LoopGui to run three acquisitions.
The following should have happened:
The traces are displayed sequentially in the 2 acquisition windows
You should have heard the shutter opening (if applicable)
The traces are saved (as indicated by three new 'saved data' messages in the Matlab Command window)
02-Dec-2007 17:46:30 - Saved data to 'C:\Data\Gordon\cells\gs0001\gs0001AAAA0026.xsg'.
02-Dec-2007 17:46:33 - Saved data to 'C:\Data\Gordon\cells\gs0001\gs0001AAAA0027.xsg'.
02-Dec-2007 17:46:36 - Saved data to 'C:\Data\Gordon\cells\gs0001\gs0001AAAA0028.xsg'.
>>
In the XSG the acquisition number increments by three to ready itself for the next acquisition
Defining a hotswitch
Pull up the hotswitch and the hs_config programs. hs_config allows you to capature the configurations of individual programs to define a particular experiment. The hotswitch, once configured, allows you to rapidly get back to the relevant experimental settings. Defining a hotswitch is moderately cumersome; but this is an extremely powerful feature that is at the core of Ephus functionality.
First enter a hotswitch name (i.e. shutterCycle1). THen make a new folder in the hotswitches directory ('shutterCycle1') which will contain all of the relevant program configurations.
Now go through the program list and 'capture' the settings for all of the programs that matter – - not more or less. More might lead to slow response times; less would likely result in errors.
In this case we capture:
ephys
acquirer
loopGui
stimulator
...