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ScanImage 3.5 is very similar to 3.0 and new users may want to refer to the [ScanImage r3.0 Documentation] to become familiar with this software. 

h4.

 Changes

However, some important changes have been made. New and returning users should familiarize themselves with the documentation of the [Important Changes in ScanImage 3.5|ScanImage r3.5.x Documentation#Important Changes in ScanImage 3.5]. Notably:

* The Selection Dialog no longer appears at startup

* The handling of the INI file, which must be created  by the user, has changed somewhat.

 
* The board wiring required for trigger and clock synchronization has been modified

* The Main Controls window has several modifications

h4. New Features

In addition to these changes, several new features have been added to ScanImage 3.5 to help support ongoing experiments by ScanImage users at HHMI/Janelia Farm Research Campus. These and other [Important New Features in ScanImage 3.5|ScanImage r3.5.x Documentation#Important New Features in ScanImage 3.5] are described fully in the documentation:

* Live RGB channel 'merge' display during Focus mode

* Unlimited & uninterrupted acquisitions via TIF streaming-to-disk

 
* Ability to accept external trigger, using separate trigger input terminal (e.g. facilitates _Ephus_\-driven acquisitions)

* Enhanced MP-285 interface and configuration

* Improved support for case of 3 'beams' (i.e. 3 Pockels Cells)

* Tools to facilitate beam and scan alignment

* Enhanced 'hot-key' functionality to support rapid program control and configuration switching

h4. Bug Fixes

A handful of bugs identified in ScanImage 3.0 have been addressed in this release.

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* The 'pixel time' and 'bin factor' parameters now update correctly following changes to the 'Fill Fraction' or 'ms/line' parameters.

* _Power Box

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** 'Constrain to Line' functionality is actually turned off at startup, matching the startup checkbox setting in the GUI

** Selecting 'Constrain to Line' no longer causes Power Box to 'shift' each time

* User preference to display grid on channel windows now correctly saves

* Prompted entry of file 'base name' actually

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h4. About Upcoming Releases
Version 3.6 is expected to be released soon after this version, supporting bidirectional and an improved sawtooth scanning user interface.