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Composition Setup

Excerpt

ScanImage

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3.6.1

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is

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a

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recommended service

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pack

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release,

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with

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important enhancements/bugfixes.

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Note
titleMind your Versions!

The Matlab and National Instruments DAQmx version compatibility guide are requirements, not recommendations. Problems have been encountered using versions newer than those listed.

Enhancements

  • Allow 2048 pixels/line
  • All graphical interaction tools (line scan/ROI/power

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  • box

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  • selection)

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  • now

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  • use

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  • new

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  • functions

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  • (independent

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  • of

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  • Image

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  • Processing

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  • toolbox)

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  • with

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  • improved

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  • stability

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  • and

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  • ability

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  • to

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  • cancel,

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  • using

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  • escape/delete.

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Bugfixes

  • Power Box 'OUT'

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  • value

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  • now

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  • works

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  • Error

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  • when

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  • parking

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  • laser

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  • for

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  • case

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  • of

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  • 2

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  • or

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  • more

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  • beams

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  • is

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  • fixed
  • 'Discard Flyback Line' option could cause errors in makeFrameByStripes() for certain scan parameters
  • The 'z'

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  • hotkey

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  • (which

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  • goes

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  • to

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  • zero

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  • motor

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  • position)

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  • no

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  • longer

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  • has

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  • effect

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  • if

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  • a

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  • relative

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  • origin

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  • has

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  • not

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  • been

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  • set

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  • Histogram/Statistics

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  • features

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  • are

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  • working

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  • again

ScanImage 3.6.0

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README

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(12-08-2010)

Panel
Include Page
ScanImage r3.6 README
ScanImage r3.6 README
ScanImage 3.6 has been developed primarily to support fast raster scanning -- bidirectional scanning, in particular, as well as faster sawtooth scanning -- providing a 4x increase in the line scan rate compared to ScanImage 3.5 and earlier versions (*0.5ms/line* vs *2ms/line* typically used in earlier versions). ScanImage 3.6 makes significant changes to core scanning functionality. There are both changes at the user interface level (the *CONFIGURATION* dialog is completely revamped) as well as under the hood. Users familiar with earlier releases should expect a short learning period to become familiar with the new scan parameters and user interface. ScanImage 3.6 adds significant performance enhancements to the 'streaming' functions -- those which are executed as _callbacks_ to process incoming data during live acquisition. As a result, display/processing/disk-logging can be sustained for high frame rate imaging, up to >60fps, using readily available and inexpensive computer hardware. ScanImage 3.6 removes several features that were visible in earlier versions: _PowerBox Stepper_, _ROI Cycler_, _UncagingMapper_, and _Custom Timings_. These specialty features have not been widely used/tested to include with the current release. They may be recapitulated in the future. In addition, ScanImage 3.6 disables Cycle mode operation, which provides ability to program stage movements between acquisitions. However, Cycle mode operation has been restored and tested in ScanImage 3.5.1, which is being co-released with ScanImage 3.6. It is planned to add Cycle mode operation back to a future ScanImage release. {comment} A full list of changes, new features, and bugfixes is presented below. has been developed primarily to support ScanImage users who no longer have ready access to the older Matlab version for which earlier ScanImage releases had been developed. ScanImage 3.5 is known to be compatible with Matlab 2007b. In addition, it is now compatible with the newer National Instruments driver (DAQmx), rather than the Traditional NI-DAQ driver required by earlier ScanImage releases. ScanImage 3.5 is very similar to 3.0 and new users may want to refer to the [ScanImage r3.0 Documentation] to become familiar with this software. h4. Changes However, some important changes have been made. New and returning users should familiarize themselves with the documentation of the [Important Changes in ScanImage 3.5|ScanImage r3.5 Documentation#Important Changes in ScanImage 3.5]. Notably: * The Selection Dialog no longer appears at startup * The handling of the INI file, which must be created by the user, has changed somewhat. * The board wiring required for trigger and clock synchronization has been modified * The Main Controls window has several modifications h4. New Features In addition to these changes, several new features have been added to ScanImage 3.5 to help support ongoing experiments by ScanImage users at HHMI/Janelia Farm Research Campus. These and other [Important New Features in ScanImage 3.5|ScanImage r3.5 Documentation#Important New Features in ScanImage 3.5] are described fully in the documentation: * Live RGB channel 'merge' display during Focus mode * Unlimited & uninterrupted acquisitions via TIF streaming-to-disk * Ability to accept external trigger, using separate trigger input terminal (e.g. facilitates _Ephus_\-driven acquisitions) * Enhanced MP-285 interface and configuration * Improved support for case of 3 'beams' (i.e. 3 Pockels Cells) * Tools to facilitate beam and scan alignment * Enhanced 'hot-key' functionality to support rapid program control and configuration switching h4. Bug Fixes A handful of bugs identified in ScanImage 3.0 have been addressed in this release. {toggle-cloak:id=BugFixes} *View list of bug fixes...* {cloak:id=BugFixes} {panel:borderStyle=none} * The 'pixel time' and 'bin factor' parameters now update correctly following changes to the 'Fill Fraction' or 'ms/line' parameters. * _Power Box_ ** 'Constrain to Line' functionality is actually turned off at startup, matching the startup checkbox setting in the GUI ** Selecting 'Constrain to Line' no longer causes Power Box to 'shift' each time * User preference to display grid on channel windows now correctly saves * Prompted entry of file 'base name' actually pertains {panel} {cloak} h4. About Upcoming Releases Version 3.6 is expected to be released soon after this version, supporting bidirectional and an improved sawtooth scanning user interface. {comment}