Table of Contents

...

maxLevel 3

Release Schedule

ScanImage 5 will :

1. unify galvo and resonant scanning capabilities (ScanImage 3 and 4, respectively); and
2. support the use of generic resonant scanning hardware with real-time FPGA processing. 

By version 5.1, ScanImage 5 will support experiments combining resonant and galvo scanning. This will include random-access and other custom scan trajectories, suited for photostimulation and multi-site recording applications.

be released in 2014. The next version, ScanImage 2015, will add new features to ScanImage 5.

Early-access releases will be available exclusively to ScanImage 5 members.

ScanImage 5 will have  ScanImage 5 will have  customization layers for both CPU and FPGA-based processing (latter for resonant scanned imaging only) which can be used to implement online or real-time analysis, including including closed-loop experiments with  with parallel imaging and (photo)stimulus.        

Release Dates

Resonant Scanning Hardware

For resonant scanned raster imaging, the following digitizer/scanner hardware is required:

Digitizer Hardware

ScanImage 5 Resonant will use National Instruments PXI FlexRIO platform. The system consists of a: PXI chassis, FPGA Module, and digitizer module.

The NI system bundles with SI5 support include:

...

•  The PXIe-1073 is a 5-slot 'remote controller' chassis connecting to the computer via a PCIe (PCI Express) card. Both PCIe card and cable are included.
• More advanced chassis options will work as well.

...

...

• PXIe-7975R will be supported after August 2014. It offers greater processing power than 7961R, allowing more real-time image processing possibilities.
  In August 2014, NI will add support for NI 5732/4 digitizer modules with the PXIe-7975.
• PXIe-7962R will be supported (bitfiles provided), but not as extensively tested

...

NI 5732  (2 Chans/80MHz)

NI 5734 (4 Chans/120MHz) 

...

...

ScanImage 2015 will support the same scanner/digitizer hardware as ScanImage 5

...

ScanImage 5 Scanner Hardware

ScanImage 5 requires a pair of scanners (one resonant and one galvo scanner) to achieve video-rate area scanning operation. 

ScanImage 5 users can choose either:

• scanner system that combines a resonant scanner and galvo scanner

• scanner components - one resonant scanner and one galvo scanner

ScanImage 5 Scanner Systems

Sutter Instrument systems:

• Resonant Scan Box
• MOM-Resonant microscope systems

ScanImage 5 Resonant Scanner Components

Cambridge Technology components

• CRS 8Khz (part 6SC08KA012-02Y)
• CRS 12Khz (part 6SC12KA012-02Y)
• Classic CRS-8 scanner 

ScanImage 5 Galvo Scanner Components 

Any analog-driven galvo scanner component, including galvanometer-mounted mirror and servo driver circuit, can be used.

The following are typical choices from Cambridge Technology

• 6210H galvo, with 6711x or 6713x servo driver
• 6215H galvo, with 6711x or 6713x servo driver

 Class 0 servo topology is recommended, but not required

...

ScanImage 5 DAQ Hardware

ScanImage 5 will use National Instruments PXI FlexRIO platform.

ScanImage 5 has required, user-selected, and user-optional DAQ components

• PXIe-1073 chassis/controller (or higher)

• PXIe-6341 X DAQ board (or higher)

User-Selected DAQ Components 

Digitizer Module

FPGA Module

• Basic Option: PXIe-7961R
• Performance Option: PXIe-7975R

For SI 5, there will be no difference in operation. 

Features in future versions (SI 2015 & beyond) may utilize the far
greater (~10x) image processing power of the PXIe-7975R, e.g. closed-loop experiments.

User-Optional DAQ Components 

Users can add additional boards for control of Beams (Pockels) and Piezo features.

 Beams control is recommended. Allows blanking laser during resonant scanner slowdown and galvo scanner flyback.

 Piezo control is optional. Allows for volume imaging

For each optional feature desired, select one of the following X series DAQ boards:

• PCIe-6321 - inserted into computer PCIe slot
• PXIe-6341 - inserted into PXIe-1073 chassis

 PCIe-6321 option is cheaper, but requires additional wiring and uses a computer PCIe slot

Computer Hardware

...

Scanner Hardware

For resonant scanned raster imaging, users require 1x each of:

Resonant Scanner: Cambridge Technologies CRS-8 or CRS-12 scanner. Includes control board.

Galvo Scanner: Cambridge Technologies 6210 or 6215 galvo system (typ. with 4-6mm mirror). Other analog-control galvos also supported.

Notes

CRS-12 support may not be ready by ScanImage 5 prerelease. Its support is planned by ScanImage 5 release date.

Galvo system's max slew rate can impact the frame rate achievable at larger fields-of-view. Slower slew rates require a larger number of resonant scanner line periods for galvo flyback.

Galvo Scanning Hardware

For galvo scanned raster imaging and future scan modes:

Digitizer Hardware

ScanImage 5 Galvo will support the following input digitizer options:

<div class="table-wrap"><table class="confluenceTable"><tbody><tr><td colspan="1" class="confluenceTd">USB X Series</td><td colspan="1" class="confluenceTd"><a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209071" class="external-link" rel="nofollow">USB-6351</a>, <a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209072" class="external-link" rel="nofollow">USB-6353</a>,<br/><a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209077" class="external-link" rel="nofollow">USB-6356</a></td><td colspan="1" class="confluenceTd">USB-6351/6353 recommended for no-Pockels/Pockels cases, respectively</td></tr></tbody></table></div>

Scanner Hardware

An X/Y analog-controlled galvo scanner pair is required.  Cambridge Technologies 6210 or 6215 galvo system (typ. with 3-6mm mirrors) is typical. Galvo system characteristics impact attainable line speed and performance of future scan modes.

<div class="table-wrap"><table class="confluenceTable"><tbody><tr><td colspan="1" class="confluenceTd"><p>Some users elect to set up TPLSM microscopes from a microscope hardware manufacturer. The following information is provided as a benefit to users</p><p><em>Sutter</em>: Full compatibility with ScanImage 5 resonant/galvo capabilities</p><p><em>Scientifica: </em></p>Thorlabs and Prai</td></tr></tbody></table></div>

Computer Hardware

ScanImage 5 (resonant and galvo modules) is expected to run on standard multi-core machines (e.g. iCore7) with at least 4GB RAM and at least 4 PCIe slots.

Example tested ScanImage 5 computer systems will be provided at the prerelease date.by the early-access release date.