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Table of Contents

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Release Schedule

ScanImage 5 will :

  1. unify galvo and resonant scanning capabilities (ScanImage 3 and 4, respectively); and
  2. support the use of generic resonant scanning hardware with real-time FPGA processing. 

By version 5.1, ScanImage 5 will support experiments combining resonant and galvo scanning. This will include random-access and other custom scan trajectories, suited for photostimulation and multi-site recording applications.

be released in 2014. The next version, ScanImage 2015, will add new features to ScanImage 5.

 Early-Access
Release(s) 
Public
Release
Comments

SI 5
(Resonant)

May 22 2014 (beta)SFN 2014

Recapitulate ScanImage 4 (resonant scanning) features with
more flexible and powerful NI FPGA (FlexRIO) hardware.

SI 2015
(Galvo & Multi)

SFN 2014  (beta)
& March 2015 (official)

SFN 2015New features beyond ScanImage 5, including galvo arb-scanning,
multi-scanner, and other features specified by ScanImage 5 members.

Early-access releases will be available exclusively to ScanImage 5 members.

ScanImage 5 will have  ScanImage 5 will have  customization layers for both CPU and FPGA-based processing (latter for resonant scanned imaging only) which can be used to implement online or real-time analysis, including including closed-loop experiments with  with parallel imaging and (photo)stimulus.        

Release Dates

 PrereleaseReleaseComments
ScanImage 5May 1, 2014Nov 1, 2014Recapitulate ScanImage 4 features with more flexible hardware
ScanImage 5.1Feb 1, 2015Nov 1, 2015
  • New capabilities incorporating Founding Member requests
  • Combined resonant and galvo scanning

Resonant Scanning Hardware

For resonant scanned raster imaging, both digitizer hardware and scanner hardware are required.

Digitizer Hardware

Tip

National Instruments (NI) offers Bundle ID BD25410 or ("Vidrio ScanImage 5") to configure a standard ScanImage 5 system with NI representatives, rather than purchasing separate components below. This will be less error-prone and it includes the required accessories. This bundle is provided for the convenience of ScanImage 5 users - there is no mark-up cost.

ScanImage 5 Resonant will use National Instruments PXI FlexRIO platform.
The system consists of three components: PXI chassis, FPGA Module, and digitizer module.

The NI system bundles with SI5 support include:

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  • The PXIe-1073 is a 5-slot 'remote controller' chassis connecting to the computer via a PCIe (PCI Express) card. Both PCIe card and cable are included.
  • More advanced chassis options will work as well.

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  • PXIe-7975R will also be supported, after August 2014. It offers greater processing power than 7961R, allowing more real-time image processing possibilities. In August 2014, NI will add support for NI 5732/4 digitizer modules with the PXIe-7975.
  • PXIe-7962R will also be supported (bitfiles provided), but not as extensively tested

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NI 5732  (2 Chans/80MHz)

NI 5734 (4 Chans/120MHz) 

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(tick) ScanImage 2015 will support the same scanner/digitizer hardware as ScanImage 5

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ScanImage 5 Scanner Hardware

ScanImage 5 requires a pair of scanners (one resonant and one galvo scanner) to achieve video-rate area scanning operation. 

ScanImage 5 users can choose either:

  • scanner system that combines a resonant scanner and galvo scanner

  • scanner components - one resonant scanner and one galvo scanner

ScanImage 5 Scanner Systems

Sutter Instrument systems:

ScanImage 5 Resonant Scanner Components

Cambridge Technology components

  • CRS 8Khz (part 6SC08KA012-02Y)
  • CRS 12Khz (part 6SC12KA012-02Y)
  • Classic CRS-8 scanner 

ScanImage 5 Galvo Scanner Components 

Any analog-driven galvo scanner component, including galvanometer-mounted mirror and servo driver circuit, can be used.

The following are typical choices from Cambridge Technology

(tick) Class 0 servo topology is recommended, but not required

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ScanImage 5 DAQ Hardware

ScanImage 5 will use National Instruments PXI FlexRIO platform.

ScanImage 5 has required, user-selected, and user-optional DAQ components

Tip

Stay tuned for a National Instruments ScanImage 5 configuration tool

We anticipate this may include small discounts on hardware

User-Selected DAQ Components 

Digitizer Module

FPGA Module

For SI 5, there will be no difference in operation. 

Features in future versions (SI 2015 & beyond) may utilize the far
greater (~10x) image processing power of the PXIe-7975R, e.g. closed-loop experiments.

Tip
titlePXIe-7961 loaners

The PXIe-7975R option will be supported by ScanImage 5 by August 2014.

Vidrio tentatively has access to a small pool of PXIe-7961R loaners

Loaners are intended for users who purchase a PXIe-7975R
but need to start using ScanImage 5 sooner than August. Contact membersupport@vidriotech.com if interested.

User-Optional DAQ Components 

Users can add additional boards for control of Beams (Pockels) and Piezo features.
(tick) Beams control is recommended. Allows blanking laser during resonant scanner slowdown and galvo scanner flyback.
(tick) Piezo control is optional. Allows for volume imaging
For each optional feature desired, select one of the following X series DAQ boards:

(info) PCIe-6321 option is cheaper, but requires additional wiring and uses a computer PCIe slot

 

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Computer Hardware

 

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Scanner Hardware

Tip

Sutter Instrument company offers a Resonant Scan Box component combining the required components in a sound-proof housing.

Both the Sutter standalone Resonant Scan Box and their full MOM-Resonant microscope system will be compatible with ScanImage 5.

Note that Vidrio currently recommends waiting until testing of the new Cambridge Technology scanner system is completed, by April 1 2014. See note below.

Note
titleTesting New Scanner Hardware

Cambridge Technology is recently offering a new CRS Resonant Scanner Driver board. This offers improved synhronization and is optimized for use with the maximal angle versions of their CRS scanner products.

Vidrio engineers have ordered this scanner and will test this new scanner system before April 1, 2014. (System 6SC08KA012-02Y with new 711-80159)

Prospective ScanImage 5 users are advised to wait for this testing before committing to a scanner hardware purchase.

ScanImage 5 is expected to work with classic CRS scanner systems - including the Sutter Resonant Scan Box  system - but potentially with small additional electronics or reduced scan angle.

For resonant scanned raster imaging, users require 1x each of:

Resonant Scanner: Cambridge Technologies CRS-8 or CRS-12 scanner. Includes control board.

Galvo Scanner: Cambridge Technologies 6210 or 6215 galvo system (typ. with 4-6mm mirror), with servo control board. Other analog-control galvos also supported.

Notes

(warning) CRS-12 support will not be ready by ScanImage 5 prerelease. Its support is planned by ScanImage 5 release date.

(info) Galvo system's max slew rate can impact the frame rate achievable at larger fields-of-view. Slower slew rates require a larger number of resonant scanner line periods for galvo flyback.

Galvo Scanning Hardware

For galvo scanned raster imaging and future scan modes:

Digitizer Hardware

ScanImage 5 Galvo will support the following input digitizer options:

FamilyModel Number(s)Comments
PCIe X SeriesPCIe-6351, PCIe-6353PCIe-6351/6353 recommended for no-Pockels/Pockels cases, respectively
PXIe X SeriesPXIe-6356, PXIe-6358PXIe boards fit into chassis used for resonant scanning (if applicable). PXIe-6356/6358 recommended for no-Pockels/Pockels cases, respectively.
S SeriesPCI-6110Not recommended for new systems, but will remain supported. A PCI slot and full-size computer case is required to house this board.

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<div class="table-wrap"><table class="confluenceTable"><tbody><tr><td colspan="1" class="confluenceTd">USB X Series</td><td colspan="1" class="confluenceTd"><a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209071" class="external-link" rel="nofollow">USB-6351</a>, <a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209072" class="external-link" rel="nofollow">USB-6353</a>,<br/><a href="http://sine.ni.com/nips/cds/view/p/lang/en/nid/209077" class="external-link" rel="nofollow">USB-6356</a></td><td colspan="1" class="confluenceTd">USB-6351/6353 recommended for no-Pockels/Pockels cases, respectively</td></tr></tbody></table></div>

Scanner Hardware

An X/Y analog-controlled galvo scanner pair is required.  Cambridge Technologies 6210 or 6215 galvo system (typ. with 3-6mm mirrors) is typical. Galvo system characteristics impact attainable line speed and performance of future scan modes.

 

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<div class="table-wrap"><table class="confluenceTable"><tbody><tr><td colspan="1" class="confluenceTd"><p>Some users elect to set up TPLSM microscopes from a microscope hardware manufacturer. The following information is provided as a benefit to users</p><p><em>Sutter</em>: Full compatibility with ScanImage 5 resonant/galvo capabilities</p><p><em>Scientifica: </em></p>Thorlabs and Prai</td></tr></tbody></table></div>
 

Computer Hardware

ScanImage 5 (resonant and galvo modules) is expected to run on standard multi-core machines (e.g. iCore7) with at least 4GB RAM and at least 4 PCIe slots.

Example tested ScanImage 5 computer systems will be provided at the prerelease dateby the early-access release date.