See also FAQs
Important items for assembly
Update for MotorizedMirrorCoverAssby, including latches to hold together: MotorizedMirrorCoverAssby_PartsList_Updated20160812.xls. See Modification & updates page.
Additional partslist additional_parts_071316.xlsx
Essential items partslist for 2p-RAM - NEW; click this 2p-RAM Parts List (072616).xlsx
The excel spreadsheet contains several sections related to the 2-photon microscope (all accessory stuff, such as wide-field imaging, optogenetics, et) is under accessory partslist, below.
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You should have ordered items 2-6 (long lead-time itmes from Thorlabs and Jenoptik).
Now order all other items (lines 7-120) EXCEPT line item 66 (Equipment Solutions; this refers to a critical part of the z-focusing mechanisms and we are trying to converge on an even faster design).
Order quantities as indicated in the spreadsheet (sometimes these diverge from the quantities indicated in the quotes)
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Standard hardware. This refers to standard mounting hardware (screws, nuts etc; the list is non-exhaustive).
Accessory partslist - NEW - 2p-RAM Accessory Optics Parts List.xlsx
This partslist includes useful items for wide-field imaging, laser scanning photostimulation (optogenetics), and features to align the imaging window to the microscope beam axis.
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| WHAT | Company | Quote | Comments |
|---|---|---|---|
| Cover | Deperini | Deperini Updated Quote Accessory Optical System 03180955 HHMI.pdf | |
| Cover | Zera | Zera Quote 1809.pdf | |
| Microscopy cube | Zera | Zera Quote 1824.pdf | |
| Camera | Point grey | POINT GREY - HHMI - Quote (20005017).pdf | For wide-field imaging |
| Custom adapters | Dysinger | Dysinger Quote 10263R.pdf | |
| Scan mirrors and drivers | Cambridge Technologies | CambridgeTech_QU160330gt HHMI 6SD10996.pdf | For laser scanning photostimulation |
PMT Shutters Modification
This modification allows the addition of shutters to each PMT channel, placed right in front of the PMT. They have a ~10 ms opening transit time, and should block very little detection light while open (not more than a percent or two).
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Shutter and driver purchase quote: This quote is for the uni-stable (normally closed) shutter. This may no longer be available, except with a minimum qty order (recent change announced on Vincent website). If so, we think that the bistable version (NS25B1T0L-VED) should fit OK and work fine. It might actually open faster, so may be preferrable overall.
Modification instructions: The asphere lens can be removed from the Thorlabs lens tube mount and mounted in the new part. Once the shutter is mounted, the wires will need to be soldered to the connector provided with the VED24 driver. Shutter driver settings note:
The settings for an NS25S type shutter operating with a VED24 driver are as follows, for the ‘Function Switches’ and ‘Pulse Duration’: Switch 1 = Up, Switch 2 = Down, Switch 3 = Down, Switch 4 = Up, & Pulse Duration = 4 [[[Switch 1 = LOCAL (‘REMOTE’ is down), Switch 2 = 18VDC, Switch 3 = Uni-stable Mode, Switch 4 = Pulse Set 1, & Pulse Duration = 20msec]]]
Shop drawings for new part and two part modifications.
Wire cap fabrication files: Use silicone, tape, or make nice wire caps using rapid prototyping method of choice. Included are STEP file, STL file (for 3d printing), or DXF file (for laser-cut acrylic).
Preprint describing the 2p-RAM
SofroniewFlickingerEtAl2016.pdf
Model files of entire system
2p-RAM DWFx file, which contains metadata for each part. This is viewed in free Autodesk Design Review software, which allows measurements.
2p-RAM STEP file, which can be opened by most CAD programs.