See also FAQs
Important items for assembly
Update for MotorizedMirrorCoverAssby, including latches to hold together: MotorizedMirrorCoverAssby_PartsList_Updated20160812.xls. See Modification & updates page.
Additional partslist additional_parts_071316.xlsx
Partslist - NEW
Essential items partslist for 2p-RAM - NEW; click this
2p-RAM Parts List (072616).xlsx
The excel spreadsheet contains several sections related to the 2-photon microscope (all accessory stuff, such as wide-field imaging, optogenetics, et) is under accessory partslist, below.
Core instrument.
You should have ordered items 2-6 (long lead-time itmes from Thorlabs and Jenoptik).3-18-2016).xlsxOrder everything under core instrument, except line item 66 (Equipment Solutions)
Order quantities as indicated in the spreadsheet (sometimes these diverge from the quantities indicated in the quotes)
To get discounts these items should be ordered by the end of April. Please try to get the orders in as soon as you can.
Refer to these quotes: (please let us know if something does not seem right!
| What | Company | Quote | For discount |
|---|---|---|---|
| Custom 2p-RAM optics | Jenoptik | JENOPTIKS - Q15633 Rev B HHMI.PDF | Long lead-time |
| Custom gantry microscope mechanics | Thorlabs | THORLABS - Quote 10004.pdf | Long lead-time |
| Various 2p-RAM parts | Thorlabs | Thorlabs Quote 10009 Mesoscope parts 3.18.16.pdf | Order by 4/30 |
| Scanners and accessories | Cambridge Technologies | Cambridge Technologies 1 System Qu031816rhCRS1262406231HP.pdf | Order by 4/30 |
| Primary dichroic | Alluxa | ALLUXA - Q210-3158 rev2 - Alluxa -HHMI, 750 SP Dichroic GDD, 1-21-2016.pdf | Order by 4/30 |
| Dichroics and filters | Semrock | Semrock Update Quote 26788 HHMI 3.17.16.pdf | Order by 4/30 |
| Custom lens | Brighten optics | BRIGHTEN OPTICS - Quotation Q20160127HHMIrev1.pdf | Order by 4/30 |
| Custom condensor lens | IRD - e-mail quote | - Quote to Janelia for P N J002981B. msg | Order by 4/30 |
| PMTs | Hamamatsu | HAMAMATSU - H10770PAH11706P-40 SEL (Example for HHMI).pdf | |
| Prism pair compressor | PLX | PLX - Quote 17365.pdf | Order by 4/30 |
| Custom optomechanics | Unique | Unique Fabrication - Quote blank # 172.docUnique Fabrication - Quote blank # 172-2.doc | Order by 4/30 |
| Custom optomechanics | Zera | Order by 4/30 | |
| Custom optomechanics | 5th axis | 5th Axis - Quote 16638 RFQ.pdf | Order by 4/30 |
| Custom optomechanics | De Perini | DePerini Updated Quote Core Microscope 03171105 HHMI 3.17.16.pdf | Order by 4/30 |
| Optomechanics | Newport | Newport Quote QTE00008655-02 (002) 3.17.16.pdf | Order by 4/30 |
| Dispersing Prism | Precision Optical | Precision_Optical_Dispersing_Prism.pdf | Order by 4/30 |
| LFA Mount Top & Bottom, Hard Stop for Stage | Zera | Zera Quote 1894 (002).pdf | Order by 7/31 |
| Voice Coil/Stage | Equipment Solutions | HHMI_Affilliate_7a16.pdf | Order by 7/26 |
| Accessory Holder | Unique Tools | Will need to obtain individual quotes; send drawing with request: J005700A.pdf | Order by 7/31 |
Preprint describing the 2p-RAM
SofroniewFlickingerEtAl2016.pdf
More info
Step file showing the gantry system - out of date
JRC supplied parts. This are parts are manufactured at Janelia. You will be billed for them.
User supplied parts. Get what you don't already have. You will need Pockels cells, shutters, some table topics, diagnostic hardware. You need to power the PMTs and also signal condition properly. We provided some commercial and home-built solutions.
Also, it will be hard to run the scope without the ScanImage 2016 bundle. Contact Bruce Kimmel <bruce@vidriotech.com> about ScanImage.
Standard hardware. This refers to standard mounting hardware (screws, nuts etc; the list is non-exhaustive).
Accessory partslist - NEW - 2p-RAM Accessory Optics Parts List.xlsx
This partslist includes useful items for wide-field imaging, laser scanning photostimulation (optogenetics), and features to align the imaging window to the microscope beam axis.
More information here.
Unless you have your own solutions in mind you will likely want to get the wide-field imaging and alignment stuff.
| WHAT | Company | Quote | Comments |
|---|---|---|---|
| Cover | Deperini | Deperini Updated Quote Accessory Optical System 03180955 HHMI.pdf | |
| Cover | Zera | Zera Quote 1809.pdf | |
| Microscopy cube | Zera | Zera Quote 1824.pdf | |
| Camera | Point grey | POINT GREY - HHMI - Quote (20005017).pdf | For wide-field imaging |
| Custom adapters | Dysinger | Dysinger Quote 10263R.pdf | |
| Scan mirrors and drivers | Cambridge Technologies | CambridgeTech_QU160330gt HHMI 6SD10996.pdf | For laser scanning photostimulation |
PMT Shutters Modification
This modification allows the addition of shutters to each PMT channel, placed right in front of the PMT. They have a ~10 ms opening transit time, and should block very little detection light while open (not more than a percent or two).
Shutter and driver purchase quote: This quote is for the uni-stable (normally closed) shutter. This may no longer be available, except with a minimum qty order (recent change announced on Vincent website). If so, we think that the bistable version (NS25B1T0L-VED) should fit OK and work fine. It might actually open faster, so may be preferrable overall.
Modification instructions: The asphere lens can be removed from the Thorlabs lens tube mount and mounted in the new part. Once the shutter is mounted, the wires will need to be soldered to the connector provided with the VED24 driver. Shutter driver settings note:
The settings for an NS25S type shutter operating with a VED24 driver are as follows, for the ‘Function Switches’ and ‘Pulse Duration’: Switch 1 = Up, Switch 2 = Down, Switch 3 = Down, Switch 4 = Up, & Pulse Duration = 4 [[[Switch 1 = LOCAL (‘REMOTE’ is down), Switch 2 = 18VDC, Switch 3 = Uni-stable Mode, Switch 4 = Pulse Set 1, & Pulse Duration = 20msec]]]
Shop drawings for new part and two part modifications.
Wire cap fabrication files: Use silicone, tape, or make nice wire caps using rapid prototyping method of choice. Included are STEP file, STL file (for 3d printing), or DXF file (for laser-cut acrylic).