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Make sure your electrophysiology amplifiers are turned on and configured. For the MultiCLamp this includes running MultiClamp Commander and the MultiClamp Telegraph client.
All other hardware should be configured using the startup initialization file (e.g. startEphussee ephusInit_model.m or sample files). Study this file carefully and make sure all configurations are correct.
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Start the program by running your hardware configuration file (e.g. startEphus or kephus)ephus.m and selecting your initialization file when prompted, or by passing the name of your initialization file to the ephus.m function at the command-line.
This brings up all Ephus program windows. When prompted for a configuration select 'cancel'; after all you don't have one yet. Make the Matlab command window visible. You should see a bunch of messages, concluding with 'loading completed'.
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The ScopeGUI is a specialized program for whole-cell recordings. It is primarily used to hunt for cells and to check cell parameters. With a model cell configured, run the scope to verify that Ephus communicates via the amplifier. Start and stop the scope. Change the gain and mode settings on your amplifier to test the connection between Ephus and the amplifer. Changing the mode settings in MultiClamp Commander should change the modes indicated in the scopeGUI.
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It is important that the scopeGUI is turned off before proceeding with other data acquisition. Otherwise an error will occur!
Program GUIs
Have a look at the menu bar of the XSG GUI. This menubar is shared by most other GUIs except programs related to the Oscilloscope. You can always use the 'Programs' menu to reactivate programs by clicking them.
Here are screenshots of the Mapper GUI, showing the 'File' and 'Programs' menus.
In the 'File' menu, above the separator we use 'Open' to configure GUI-related files (e.g. pulse directory for the pulseEditor; see below)
Below the separator is the ProgramManager menu item - which is used to save and load Ephus configurations.
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In the pulseEditor, under pulseSets hit 'New'. The program will prompt you for a pulse set name. Type in 'basicEphys'.
Then make your first pulse by hitting 'New' in the Pulses panel; choose Normal. Name it testStep and pick give it some parameters as shown in the Figure (Number=1; ISI=100; width=100; amp=-5; delay=100). Select To view the pulse, select 'Plot' if you'd like to view the pulse. (modify the Display Time if necessary).
Now we want to apply this pulse (a -5mV voltage step) during a whole cell recording using a test cell. In the ephys window select Stim On and Acq On. Select the pulse set and pulse using the drop-down menus. Set the trace length in seconds (i.e. 1 second). (Remember to turn off the scopeGUI; see warning above). Press 'Start' to collect your first electrophysiology trace. (If multiple channels are listed in the Ephys GUI 'Amplifier' listbox, you may need to select a pulse set and pulse for all channels, even if Stim On and Acq On are unchecked.)
The following happens:
The trace is displayed in the acquisition window
The trace is saved (as indicated in the Matlab Command window)
In the XSG the acquisition number increments by one to ready itself for the next acquisition
Running a looped data acquisition
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