SECTION 1: INITIAL MODULE ASSMBLEY
These modules should be assembled on a bench before mounting on the vertical breadboard. Assembly of the modules can be done in any order.
Entrance beam expander: EntranceBeamExpander_4x1
1) Mount the Thor_ACN254-050-B:1 lens in its lens tubes ensuring that its curved sides will point inwards when assembled.
2) Assemble the cage from thorlab parts including Thor_AC254-200-B-ML mounted lens. Set the lens cage plates approximately 141mm apart. The exact position of the plates will be set during microscope assembly.
Remote focus mirror positioner: RFAMirrorAssemb_EquipSolLFA2004
1) Gather parts for mounting the mirror on the voice coil bobbin. Clean the mirror mounting screw 90666A101_TYPE 316 SS LOW PROFILE SOCKET HEAD CAP SCREW with drop of methanol. Place small drop (30 ul) of lock-tight onto threads on the voice coil bobbin. Screw in screw, and leave overnight to set.
2) Glue remote focus mirror onto the screw using 1-day epoxy. Fill hole on top of screw with epoxy and then mount mirror centered on the screw head (slight deviations from the center of the screw head are fine).
3) Assemble the voice coil base and mount voice coil
***INSERT PHOTO OF FINISHED ASSEMBLY***
Fold mirror assembly: FoldMirrorAssemb
1) Attach gimbal mount to translation stage via adaptor plate.
2) Adjust thickness of gimbal mount to 29.7 mm. The knob in the bottom corner should no longer be adjusted. Mark it with a red X to ensure it doesn't accidentally get changed during the setting of the compensators.
***INSERT PHOTO OF RED X on gimbal***
3) Cement the fold mirror to the gimbal.
***Describe gluing procedure ***
***INSERT PHOTO OF FINISHED ASSEMBLY***
Imaging objective tray: IMOTrayAssby
1) Set to of plunger to be flush with the metal surface of the tray
2) Slide dowel rod in place
3) Assemble latch, making sure orientation is correct when the latch is fully closed and open. The exact height of the latch will be set during final assembly.
Big detection lens and accessory optics dichroic holder: BigDetLensHolderAssby
DichroMotorBaseAssby
Assembling base plate, screw in bearings, make sure balls do not fall out of carridge
Glue magnets into base plate - make sure poles face in same direction **** INCLUDE PHOTO ***
Glue in rack with 5 min epoxy, push down hard so flush
MovingDichroAssby
Assembling slider & carriage
Motor assembly
Solder motor wires to carriage connector
Mounting of accessory dichoric / mirror (gluing to spider)
BigDetLensHolderOnlyAssby
Mounting slider in holder
Mounting lenses (big collection lens and accessory tube lenses)
MotorizedMirrorCoverAssby
Assemble slider cover
Emission splitting dichroic assembly: EmissSplitDichroAssby
Assembling the breadboard periscope
Creating the remote focus objective assembly
Gluing the polarizing beam splitter to the remote focus objective assembly
Mounting the quarter waveplate
Mounting the remote focus objective
Resonant mirror chamber
Put cable in with the epoxy
Assemble resonant mirror stage and actuator
Assemble alignment jig
Mount resonant mirror in mount using alignment jig
Place resonant mirror into chamber
Assembling the lid with the o-ring and tightening
Virtually conjugate galvo-galvo pair
Build alignment jig
Mount galvo mirrors on shafts
Setup alignment laser
Wire up and turn on galvos
Do alignment
Assemble the V-blocks
For pupil relay 1,2 and 3
Imaging objective mounting
Assemble imaging objective mount
Gluing kinematic mount
Assembling primary dichroic cap
Putting in spring plungers and set screws
Emission splitting dichroic assembly
PMT assembly
Assemble alignment jigs
Alignment jig cage assembly
Imaging objective alignment jig mount
Assembling the covers
Assembling objetive mounted optic holder
Accessory optics
1) Remove galvo mirrors from mount
Mounting galvo driver and resonant driver boards (Spencer / Steve documentation)
Assembling compressor
Assemble the retroreflector carriage
1) Mount the AP90RL large angle bracket on the XT95P11 carriage.
2) Attach the TSX-1D stage to the AP90RL large angle bracket.
3) Use two M6 12mm screws to attach Thor_BA2F to retroreflector.
4) Attach the RS1:4 to the TSX-1D stage with a set screw.
5) Slide the BA2F onto the RS1:4. Adjust the orientation such that when the beam enters the retroreflector it will be reflected up without hitting an edge.
Assemble the 2x beam expander
1) Attach the SM1D12D ring iris to the GBE02-B 2x beam expander, making sure it is on the correct end.
2) Attach the SM1RC lens tube rings to the TR-05:4 posts and put them in the UPH3 universal post holders.
3) Slide the beam expander into the lens tube rings and tighten them
Assemble the prism pair compressor
Use norland 81 UV cure glue.
1) Glue prisms to TRT3 post with cutout. First glue the bottom prism, flush to the top surface of the post cutout, and positioned at a right angle. Put a few drops of UV cure glue on post and place prism on top. Do this inside a V-block to ensure the angles are correct. Once the bottom prism has been glued down, glue to top prism at a distance of 39.3mm (between outside faces). Then glue mirrors to prisms. Hold post in vice at correct orientation, place UV cure glue on prism and glue down mirror. Rotate post to glue second mirror.
2) Glue mirror and prism to polaris mirror mount, making sure orientation is correct. Attach this mirror mount to the top of the KB1X1 kinematic mount using a 8-32 screw and a nut.
3) Glue two mirrors and prisms to polaris mount and centering plate. First attach KCP2 centering plate to polaris mount. Glue one prism directly to the mount, then glue one prism to the the side of the centering plate. Leave a 1 mm space between the two prisms, and a 1 mm gap between the back face of the prism and the mount. Make sure the side of the prisms are both aligned to each other and the side of the polaris mount.
4) Attach the TRT3 post of the top plate of the KB1X1 kinematic mount using an AE8E25E internal to external thread adapter. The length of the external thread adapter needs to be shortened by about two threads to let it fit inside the TRT3 post.
5) Attach one polaris mount to the RM1F cube. Attach this cube with the RS2M spacer to the BA2 baseplate, making sure the cube is orientated such that the corner of the polaris mount is over the center of the BA2. Attach the BA2 to the Optosigma rotation stage, sliding the BA2 so the end of the slots is flush against the screws.
6) Secure the optosigma to the breadboard such that it is 25.3 mm from right side and 2.5 mm from top side.
7) Secure posts with polaris mounts to breadboard, matching the location of the items with their approximate locations in the breadboard. Ensure that the mirror, prisms all lie on the same axis.
8) Set the height of the KMCP to be 46.0 mm above the breadboard.
9) Glue custom prism onto polaris mount. Use norland 81. There are two polished and one un-polished side. Note prism is not an equilateral triangle. Make one side flush with polaris mount, and tip flush with other edge of mount.
Making samples
Beads
Fluorescein
Accessory optics
SECTION 2: MOUNT ON BREADBOARD
Mounting on the breadboard
SECTION 3: WIRING
Set up scanimage
Machine data file setting
Wiring galvos / voice coil / PMTs / etc.
SECTION 4: TABLE OPTICS ALIGNMENT
Initial table optics
Pockels cell
Shutter
2x table beam expander
Assembling the compressor
Build enclosure for table optics
SECTION 5: MICROSCOPE ALIGNMENT
Multistage periscope alignment
Quarter wave plate adjustment for multistage periscope
Alignment of beam to remote focus objective
Adjustment of quarter wave plates for maximum transmission
Alignment of RF mirror assembly
Alignment of beam to imaging objective using compensators
Adjustment of galvo voltage divider gain
SECTION 6: IMAGING
Dye pool imaging to set compensators
Dye pool imaging to set retroreflector position
Image beads and measure PSF
APPENDIX A:
Accessory optical path
Assembly, alignment, calibration, adjustment