Should I order all items on the Jenoptik quote?

No. Only order items 1-5. Note in addition that pupil relays 1 & 2 are identical (but you need 2), so it's only 4 distinct parts. 

We decided to order items 6-8 from another vendor to save some $$.

 

After Jenoptik and Thorlabs Gantry, what is the longest lead-time item?

Cambridge Technologies scan mirrors. These can take 3 months, so order them as soon as you can.

 

Should I order everything on '2p_RAM Parts List (3-18-2016)'?

All but:

Do not yet order Line 66. This is the voice coil driven mirror that implements z-focusing. We are still working with the manufacturer to make it smaller and even faster. Stay tuned.

JRC Supplied parts: These will be made in the Janelia shops. You will be billed.

User supplied parts: This depends on what you have in the lab. Use your judgment. The microscope will be hard to use without the ScanImage 5 bundle. The other stuff should be self-explanatory.

 

There is a mismatch in the numbers in the quotes and in '2p_RAM Parts List (3-18-2016)'?

This is because we asked vendors to quote quantities of 5 & 10 in cases where discounts are possible.
Please order the number of units specified in '2P_RAM Parts List (3-18-2016); refer to the quote.

What about the list '2p-RAM Accessory Optics Parts List'?

This pertains to the auxillary light path (not two-photon). It is a collection of parts, mostly off-the-shelf, that provide very useful functions.
These will allow optogenetics, wide-field imaging, and alignment of the coverglass to be normal to the beam axis.