SECTION 1: INITIAL MODULE ASSMBLEY
These modules should be assembled on a bench before mounting on the vertical breadboard. Assembly of the modules can be done in any order.
Entrance beam expander: EntranceBeamExpander_4x1
1) Mount the Thor_ACN254-050-B:1 lens in its lens tubes ensuring that its curved sides will point inwards when assembled.
2) Assemble the cage from thorlab parts including Thor_AC254-200-B-ML mounted lens. Set the lens cage plates approximately 141mm apart. The exact position of the plates will be set during microscope assembly.
Remote focus mirror positioner: RFAMirrorAssemb_EquipSolLFA2004
1) Gather parts for mounting the mirror on the voice coil bobbin. Clean the mirror mounting screw 90666A101_TYPE 316 SS LOW PROFILE SOCKET HEAD CAP SCREW with drop of methanol. Place small drop (30 ul) of lock-tight onto threads on the voice coil bobbin. Screw in screw, and leave overnight to set.
2) Glue remote focus mirror onto the screw using 1-day epoxy. Fill hole on top of screw with epoxy and then mount mirror centered on the screw head (slight deviations from the center of the screw head are fine).
3) Assemble the voice coil base and mount voice coil
***INSERT PHOTO OF FINISHED ASSEMBLY***
Fold mirror assembly: FoldMirrorAssemb
1) Attach gimbal mount to translation stage via adaptor plate.
2) Adjust thickness of gimbal mount to 29.7 mm. The knob in the bottom corner should no longer be adjusted. Mark it with a red X to ensure it doesn't accidentally get changed during the setting of the compensators.
***INSERT PHOTO OF RED X on gimbal***
3) Cement the fold mirror to the gimbal.
***Describe gluing procedure ***
***INSERT PHOTO OF FINISHED ASSEMBLY***
Imaging objective tray: IMOTrayAssby
1) Set to of plunger to be flush with the metal surface of the tray
2) Slide dowel rod in place
3) Assemble latch, making sure orientation is correct when the latch is fully closed and open. The exact height of the latch will be set during final assembly.
Big detection lens and accessory optics dichroic holder: BigDetLensHolderAssby
DichroMotorBaseAssby
Assembling base plate, screw in bearings, make sure balls do not fall out of carridge
Glue magnets into base plate - make sure poles face in same direction **** INCLUDE PHOTO ***
Glue in rack with 5 min epoxy, push down hard so flush
MovingDichroAssby
Assembling slider & carriage
Motor assembly
Solder motor wires to carriage connector
Mounting of accessory dichoric / mirror (gluing to spider)
BigDetLensHolderOnlyAssby
Mounting slider in holder
Mounting lenses (big collection lens and accessory tube lenses)
MotorizedMirrorCoverAssby
Assemble slider cover
Emission splitting dichroic assembly: EmissSplitDichroAssby
Assembling the breadboard periscope
Creating the remote focus objective assembly
Gluing the polarizing beam splitter to the remote focus objective assembly
Mounting the quarter waveplate
Mounting the remote focus objective
Resonant mirror chamber
Put cable in with the epoxy
Assemble resonant mirror stage and actuator
Assemble alignment jig
Mount resonant mirror in mount using alignment jig
Place resonant mirror into chamber
Assembling the lid with the o-ring and tightening
Virtually conjugate galvo-galvo pair
Build alignment jig
Mount galvo mirrors on shafts
Setup alignment laser
Wire up and turn on galvos
Do alignment
Assemble the V-blocks
For pupil relay 1,2 and 3
Imaging objective mounting
Assemble imaging objective mount
Gluing kinematic mount
Assembling primary dichroic cap
Putting in spring plungers and set screws
Emission splitting dichroic assembly
PMT assembly
Assemble alignment jigs
Alignment jig cage assembly
Imaging objective alignment jig mount
Assembling the covers
Assembling objetive mounted optic holder
Mounting galvo driver and resonant driver boards (Spencer / Steve documentation)
Making samples
Beads
Fluorescein
SECTION 2: MOUNT ON BREADBOARD
Mounting on the breadboard
SECTION 3: WIRING
Set up scanimage
Machine data file setting
Wiring galvos / voice coil / PMTs / etc.
SECTION 4: TABLE OPTICS ALIGNMENT
Initial table optics
Pockels cell
Shutter
2x table beam expander
Assembling the compressor
Build enclosure for table optics
SECTION 5: MICROSCOPE ALIGNMENT
Multistage periscope alignment
Quarter wave plate adjustment for multistage periscope
Alignment of beam to remote focus objective
Adjustment of quarter wave plates for maximum transmission
Alignment of RF mirror assembly
Alignment of beam to imaging objective using compensators
Adjustment of galvo voltage divider gain
SECTION 6: IMAGING
Dye pool imaging to set compensators
Dye pool imaging to set retroreflector position
Image beads and measure PSF
APPENDIX A:
Accessory optical path
Assembly, alignment, calibration, adjustment