These modules should be assembled on a bench before mounting on the vertical breadboard or table. Assembly of these modules can be done in any order.
Core microscope modules
Entrance quarter waveplate
Entrance beam expander
Beam periscope
Remote focus objective assembly
Remote focus mirror positioner
Mountings for pupil relays
Resonant mirror chamber
Fold mirror
Galvo scan assembly
...
Imaging objective tray
Imaging objective
Primary dichroic cap
Big detection lens and accessory optics dichroic holder
...
Detection tube
Upper detection arm base
Detection channel assembly
Covers
Accessory optical modules
Accessory optical system
Table modules
Prism pair compressor
Beam enclosure
Electronic modules
Galvo driver box
Data acquisition
PMT and assorted electronics
Imaging software
Alignment modules
sample holder that attaches to objective
Assembling primary dichroic cap
Putting in spring plungers and set screws
PMT assembly
The goal is to couple the lens onto the PMT face with oil
Seal the edge of the PMT face to the PMT housing with 5 min epoxy - careful not to soil the plate above the photocathode
Add 30 - 50 ul of high viscosity oil
Place condensor lens on oil. Make sure there are no bubbles and carefully remove overflow oil from the housing.
Place a thin ring of quick seal (WPI) on the PMT housing around the lens
Assemble alignment jigs
Alignment jig cage assembly
Imaging objective alignment jig mount
Assembling the covers
Assembling objetive mounted optic holder