Servo (cusp) delay adjustable from Main Controls
For a given scan speed (ms/Line), ScanImage should 'know' the mirror servo controller's phase delay – the time lag between the command signal and the actual miror position – in order for acquired image data to be correctly 'aligned' with the laser scan. This value is specified in ScanImage by the Cusp (Servo) Delay control. As in ScanImage 3.0, this parameter is adjusted in the Advanced Configuration... dialog box:
The Cusp (Servo) Delay control is renamed from Cusp Delay in ScanImage 3.0
As in ScanImage 3.0, the Cusp (Servo) Delay value is specified as a fraction of the Seconds / Line value displayed in the
Configuration...
dialog box (which is determined by the ms/Line and Fill Fraction controls in the Advanced Configuration... dialog)
As in ScanImage 3.0, the Configuration... dialog box, is accessed via Settings >> Edit >> Configuration... from the
Main Controls
window. The Advanced Configuration... dialog box is accessed from the Configuration... menubar at Settings >> Advanced....
ScanImage 3.5 additionally allows the Servo Delay to be adjusted via new controls which now appear on the
Main Controls
window:
New Servo Delay controls (at bottom right) allow adjustment of mirror servo controller's phase delay value, including during live (FOCUS) imaging. Selecting the Fine? checkbox causes arrow controls to increment/decrement value in fine steps.
Servo Delay value is linked to the Cusp (Servo) Delay value in the Advanced Configuration... dialog box.
Usage Notes
The effect of adjusting the Servo Delay is to shift the imaged area left or right. When the servo delay is too (small/large), a 'reflected' image will appear on the (right/left) side of the image. The optimal servo delay value is exactly between the values where these reflected images become apparent on either side.
If it is not possible to eliminate the 'reflected' image with any value of the servo delay, try reducing the Fill Fraction value in the
Advanced Configuration
dialog box.
The Servo Delay should be re-optimized if either ms/Line or Fill Fraction is modified. It is generally advised to save the three parameters together as part of a saved configuration (CFG) file. If users are frequently changing their ms/Line and/or Fill Fraction variables, the use of fast configurations is recommended.
ScanImage 3.6 will more completely address issues related to optimizing scan parameters
'Infinite' FOCUS operation
An Inf Focus checkbox has been added to the
Main Controls
window. Selecting this checkbox will cause FOCUS acquisitions to continue indefinitely until the user presses ABORT. When deselected, the ScanImage 3.0 behavior of scanning 80 frames before stopping is employed.