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October 18, 2016: Accessory optics system: Coverglass alignment system alignment issue

It's possible that the spot that the coverglass alignment system should project onto the camera for this system does not hit the camera, due to small misalignments of several optics, even when the coverglass is perfectly aligned with the imaging objective.  There are few degrees of freedom to fix this, as the assembly is currently modeled.  We fixed this by separating the LED and pinhole from the accessory optical systems main body, and attaching it to the main microscope vertical breadboard, allowing it to move in x and y relative to the rest of the system.  See picture below. 


October 13, 2016: Accessory optics system: 1p photostimulation galvo issue

The design of the standard electrical connector for the 6210H galvo changed at some point in the past, and the accessory optical system design only works with the old connectors, while the galvos purchased for the accessory optical system (XY 6210H galvos w/ 3mm aperture mirror set) have the new connector.  As such, the y-axis galvo will NOT FIT into the 2p-RAM system (it's connector hits PupilRelay3).  The easiest way to fix this problem is to purchase a replacement 6210H galvo with the old style connector, which is still available.  Gary Tillman <Gary.Tillman@cambridgetechnology.com> created system part # 6SD11390 ($677) for this, and it should contain the proper galvo, a y-axis mirror (attached to the galvo), and the proper wire to connect the galvo to the driver.  He thought that it would not be necessary to send your driver in to be retuned to the new galvo.  (It would be best to confirm all of this with Gary when you place the order.)  There IS a 10-12 week lead time to receive the galvo, however. 

If a fix is needed in less than 10-12 weeks, then there is an alternate, 2-step solution (see photo below). 

  1. Take the y-axis 6210H galvo, remove the electrical connector that is soldered to it (cut it off, and then desolder the pins one at a time), and then remove the connector on the cable that goes with the galvo and solder each wire directly to the galvo PCB board.
  2. Modify the accessory optics system main housing: the pocket that accomodates the y-axis galvo PCB board needs to be widened and made more deep, as this PCB is itself different on the newer galvo design.  The pocket should be widened to have a 39mm diameter, and should have its depth increased by 1mm.  The hole that the galvo itself goes through need not be changed.

From left to right: The old style connector.  The new style connector.  A galvo with the new-style connector that was made to fit into the old design through soldering the wire directly, and modifying the accessory optics main body part.

 

 

October 12, 2016: Resonant mirror angle alignment issue

Issues with alignment were found when using the original resonant mirror angle alignment procedure.  A new, in situ procedure was developed, which does require modifying the resonant mirror chamber part from the original design.

September 2016 and previous

NEW- Custom condensor lens from IRD

The custom condensor lens (to be attached to the PMT) is not ideal. IRD was not able to manufacture the lens without a chamfer on the stem of the mushroom, which prevents the lens to fit entirely into the PMT opening. However, the gap between condensor lens and the PMT housing is sufficiently small so that we do not consider this a problem. You may have to press the lens into the PMT opening. 

We were offered a 25% discount on this order, due to this issue.  Please check that this discount is applied on your invoice.

 

NEW- Collection of additional optional parts
These were the parts that we bought during the construction of the 2p-RAM for the workshop. In the previous partslist these were items under 'user supplied parts' (e.g. enclosure of the laser). Use your own discretion as to what you might need.
Update Aug 29: size of two mirrors have been increased.

NEW- Essential additional items for the Thorlabs kit
There are items that did not make it into the original Thorlabs kit order.

Dowel pins update

Order better dowel pins: MSC (http://www.mscdirect.com/) part # 88231998 Dowel Pins; 

(MCMASTER-CARR 90145A567 Dowel Pins are BAD)

 
Zera issues
Three pieces need fixing.

Gary Zera from Zera Development will be reaching out to all of you regarding modifying two additional parts (three in total). 

Alternatively, you can have your shop fix the pieces.

 

J003114 - Det tube

The surface that needs to be fixed is marked with the Diameter 3.055 dimension (zone C1).  The center of this curve has to be located using the dimension 2.820 (it currently is located using the dimension 2.835).

J003119a - Secondary dichroic holder 
The surfaces that need to be fixed are marked with the R.094 dimension.

J003119A.pdf


J003103 - Primary dichroic cap 

The surface that needs to be fixed is marked with the .434” dimension (zone D3 on the drawing).  See picture.  The proper datum surfaces A and B need to be used to measure this dimension correctly.

J003103.pdf





PMT issue
PMT — it looks like some of you ordered 

H10770PA-40 SEL - GaAsP without shuttering

instead of 

H11706P-40 SEL - GaAsP with shuttering

Left, H11706P-40 SE; right, H10770PA-40. 

Obviously the size of the PMTs is different. The PMT holder was designed for H11706P-40 SEL.
H10770PA-40 SEL has a smaller crossection and no mounting holes.

SOLUTION: It should be relatively easy to glue this PMT to the provided PMT holder. Simply replace the WPI Kwik-Cast with some 5 min epoxy. Make light tight with some electrical tape if necessary.

 
 

 

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